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Related Experiment Video

Updated: Jun 8, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Simultaneous detection of multiple single-nucleotide polymorphisms by a simple membrane chip.

Yu-Hsiang Yang1, Ruei-Nian Li, Shey-Cherng Tzou

  • 1Graduate Institute of Medical Genetics, Kaohsiung Medical University, Kaohsiung, Taiwan.

Genetic Testing and Molecular Biomarkers
|September 23, 2010
PubMed
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This study introduces a novel, cost-effective chip for simultaneously detecting multiple single-nucleotide polymorphisms (SNPs). This user-friendly tool accurately identifies genetic variations, aiding in disease susceptibility and therapeutic response predictions.

Area of Science:

  • Biotechnology
  • Genetics
  • Molecular Diagnostics

Background:

  • Single-nucleotide polymorphisms (SNPs) are crucial for predicting disease susceptibility and therapeutic responses.
  • Existing multi-SNP screening technologies can be costly and require specialized equipment.

Purpose of the Study:

  • To develop a cost-effective and user-friendly chip for simultaneous detection of multiple SNPs.
  • To validate the chip's accuracy against direct sequencing methods.

Main Methods:

  • Designed and blotted SNP-specific oligonucleotide probes for angiotensinogen, cholesteryl ester transfer protein, and apolipoprotein E onto a nylon membrane.
  • Utilized a dot blotting-analogous protocol for SNP detection.
  • Analyzed samples from 20 donors.

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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
10:01

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Main Results:

  • The developed chip accurately detected four specific SNPs simultaneously.
  • SNP detection by the chip showed perfect concordance with direct sequencing results.
  • The chip demonstrated robustness and user-friendliness, requiring no sophisticated instrumentation.

Conclusions:

  • The novel chip is a reliable tool for multiple SNP screening.
  • This technology has potential for future applications in identifying disease-associated genes.
  • The cost-effectiveness and ease of use make it suitable for broader clinical applications.