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Published on: April 21, 2015
[The expression of toll-like receptor4 and NF-kappaB in ulcerative colitis]
Zhen-hai Yu1, Li-dong Chen, Zhi-qiang Wang
1Department of Anatomy, Binzhou Medical College, Yantai Yu Huang Ding Hospital, Yantai, China. yt_whitewater@163.com
Aim:
To investigate the expression of TLR4 and NF-kappaB in ulcerative colitis (UC).
Methods:
Colonic biopsy specimens were collected from active UC and controls. The expression of TLR4 and NF-kappaBp 65 were analyzed by immunohistochemistry (IHC) and RT-PCR.
Results:
RT-PCR revealed a significant increase of TLR4 and NF-kappaBp 65 antigen expression in colonic mucosa of UC compared with colonic mucosa of controls (TLR4: 143.658+/-33.870, 30.531+/-8.442, t=24.253, P<0.01; NF-kappaBp65: 185.773+/-37.625, 23.810+/-7.038, t=31.664, P<0.01). IHC show that the expression of TLR4 and NF-kappaB was significantly higher in colonic mucosa of UC compared with colonic mucosa of controls.
Conclusion:
The expression of the TLR4 and NF-kappaB was increased in the colonic mucosa of UC compared with colonic mucosa of controls. It may be closely associated with the course of ulcerative colitis.
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