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A Multiplex PCR assay to differentiate between dog and red fox
M Weissenberger1, W Reichert, R Mattern
1Institute of Legal Medicine, University of Heidelberg, Voßstr. 2, 69115 Heidelberg, Germany. Melanie.Weissenberger@med.uni-heidelberg.de
Forensic Science International. Genetics
|October 2, 2010
Summary
Distinguishing between domestic dogs and red foxes is crucial for authenticating insurance claims after animal-related car accidents. Microsatellite analysis effectively differentiates these closely related species using genetic markers.
Area of Science:
- Forensic Science
- Genetics
- Wildlife Biology
Background:
- Car accidents involving wildlife, particularly red foxes (Vulpes vulpes), are common in Baden-Württemberg, Germany.
- Accurate identification of species is necessary for insurance claim authentication.
- Domestic dogs (Canis familiaris) share close genetic relation to red foxes.
Purpose of the Study:
- To develop a reliable method for differentiating between dogs and foxes for forensic casework.
- To analyze genetic variations in microsatellite loci between dogs and foxes.
Main Methods:
- Analysis of allele frequency distribution and sizes at two canine microsatellite loci (CPH4 and CPH12).
- DNA sequencing of PCR products from 26 dogs and 19 red foxes.
- Establishment of a sequenced allelic ladder for comparison.
Main Results:
- Red foxes showed a single allele at each microsatellite locus.
- Dogs exhibited 7 alleles at the CPH4 locus and 6 alleles at the CPH12 locus.
- Sequence analysis revealed distinct differences between dog and fox DNA.
Conclusions:
- Microsatellite polymorphisms provide an efficient and accurate method for differentiating between dogs and foxes.
- This genetic approach is valuable for forensic investigations involving animal-related incidents.
