Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

New targets and procedures for validating the valence geometry of nucleic acid structures.

Nucleic acids research·2026

Same author

Updated resources for exploring experimentally-determined PDB structures and Computed Structure Models at the RCSB Protein Data Bank.

Nucleic acids research·2024

Same author

Making fluorescence-based integrative structures and associated kinetic information accessible.

Nature methods·2024

Same author

Outcomes of the EMDataResource cryo-EM Ligand Modeling Challenge.

Nature methods·2024

Same author

IHMCIF: An Extension of the PDBx/mmCIF Data Standard for Integrative Structure Determination Methods.

Journal of molecular biology·2024

Same author

Community recommendations on cryoEM data archiving and validation.

IUCrJ·2024

Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026

Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026

Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026

Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026

Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026

Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

Unified data resource for cryo-EM.

Catherine L Lawson¹

¹Department of Chemistry and Chemical Biology and Research Collaboratory for Structural Bioinformatics, Rutgers, The State University of New Jersey, USA.

Methods in Enzymology

|October 5, 2010

Summary

This summary is machine-generated.

Three-dimensional cryoelectron microscopy (3D cryo-EM) reveals macromolecular structures. EMDataBank.org unifies access to cryo-EM data archives, facilitating scientific research and data sharing globally.

More Related Videos

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

gP2S, an Information Management System for CryoEM Experiments

gP2S, an Information Management System for CryoEM Experiments

Published on: June 10, 2021

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Area of Science:

Structural biology
Biophysics
Biochemistry

Background:

Three-dimensional (3D) cryoelectron microscopy (cryo-EM) is a powerful technique for determining the structures of macromolecules and their complexes.
Access to and integration of cryo-EM data are crucial for advancing structural biology.

Purpose of the Study:

To introduce EMDataBank.org as a unified resource for cryo-EM data.
To facilitate the deposition and retrieval of cryo-EM maps, models, and metadata.
To promote wider scientific community use of cryo-EM structural data.

Main Methods:

Development and implementation of EMDataBank.org.
Integration of EM Data Bank (EMDB) and Protein Data Bank (PDB) archives.
Establishment of a centralized platform for data deposition and retrieval.

Main Results:

EMDataBank.org provides a "one-stop shop" for global cryo-EM data.
The resource unifies public access to major EM structural data archives.
Facilitates accessibility and utilization of macromolecular structures determined by cryo-EM.

Conclusions:

EMDataBank.org enhances the accessibility and usability of cryo-EM structural data.
The unified resource supports collaborative research and accelerates discoveries in structural biology.
Streamlined data sharing through EMDataBank.org benefits the global scientific community.