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Updated: Jun 8, 2026
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Comparison of hydrodistillation and headspace solid-phase microextraction techniques for antibacterial volatile
Krystyna Skalicka-Wozniak1, Renata Los, Kazimierz Glowniak
1Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, 1 Chodzki, PL-20-093 Lublin, Poland. kskalicka@pharmacognosy.org
Abstract:
The volatile compounds from the fruits of Seseli libanotis, wild-growing in Poland, were obtained by hydrodistillation (HD) and headspace solid-phase microextraction techniques (HS-SPME) and analyzed using GC-MS. A total of 58 components were identified. The most abundant compounds were sabinen and beta-phellandrene. MIC (minimal inhibitory concentration) and MBC (minimal bactericidal concentration) values for the essential oil against reference bacterial strains and clinical S. aureus isolates were determined. The essential oil showed better antibacterial activity against the Gram-positive bacteria (MICs between 0.15 to 1.25 mg/mL) when compared with Gram-negative bacteria (MICs between 1.25 to 2.5 mg/mL). The low values of the MBC/MIC ratio suggest that the examined oil behaved as a bactericidal agent.
