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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

miRNA profiling on high-throughput OpenArray™ system.

Elena V Grigorenko1, Elen Ortenberg, James Hurley

  • 1BioTrove Inc, Woburn, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 9, 2010
PubMed
Summary
This summary is machine-generated.

High-throughput miRNA profiling is crucial for understanding biological processes. A novel OpenArray™ nanoplate system enables precise quantification of microRNAs (miRNAs) using real-time PCR, advancing miRNA research.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are key regulators in biological processes.
  • Accurate quantification of miRNAs is essential for physiological and pathological studies.
  • The growing number of known and predicted miRNAs necessitates advanced profiling technologies.

Purpose of the Study:

  • To describe a high-throughput system for miRNA profiling.
  • To demonstrate the utility of the OpenArray™ nanoplate for miRNA quantification.
  • To present methods for miRNA profiling using dual-label probe chemistry.

Main Methods:

  • Utilizing an OpenArray™ nanoplate for high-density, nanoliter real-time PCR assays.
  • Implementing dual-label probe chemistry (Taqman®) for miRNA detection.
  • Profiling microRNAs across various biological samples.

Main Results:

  • The OpenArray™ system supports up to 3,072 real-time PCRs per experiment.
  • Experimental data demonstrate the system's performance in miRNA quantification.
  • The technology offers high throughput comparable to microarrays and precision similar to qPCR.

Conclusions:

  • The described OpenArray™ system provides a powerful tool for comprehensive miRNA profiling.
  • This high-throughput approach facilitates the investigation of miRNA functions in diverse biological contexts.
  • The system enhances the ability to study a large number of known and predicted miRNAs efficiently.