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Experimental phaeohyphomycosis
L Pospísil1, J Bucek, M Moster
1Clinic of Dermatovenerology, Faculty of Medicine, Masaryk University, Brno, Czechoslovakia.
Abstract:
The authors performed an experimental infection of the rabbit eye with Wangiella dermatitidis which had been isolated from the corneal ulcer of a patient. The fungus was inoculated into the front chamber and the vitreous body. The disease showed a trend to spontaneous recovery. The individual phases of the experimental infection were followed by histology and electronmicroscopy, both TEM and SEM. Different stages of development of the polymorphous fungus, as sclerotic bodies, mycelial filaments and yeast-like cells, could be demonstrated.
Insights
This study shows Wangiella dermatitidis experimental rabbit eye infections tend to heal spontaneously. Histology and electronmicroscopy revealed fungal development stages during infection and recovery.
Area of Science:
- Ophthalmology
- Mycology
- Infectious Diseases
Background:
- Wangiella dermatitidis is a fungus that can cause severe eye infections.
- Understanding its pathogenesis in ocular tissues is crucial for treatment.
Purpose of the Study:
- To investigate the experimental infection of rabbit eyes with Wangiella dermatitidis.
- To characterize the fungal morphology and disease progression using advanced microscopy techniques.
Main Methods:
- Experimental inoculation of rabbit anterior chamber and vitreous body with Wangiella dermatitidis.
- Histological examination of infected ocular tissues.
- Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for ultrastructural analysis.
Main Results:
- The experimental ocular infections demonstrated a tendency towards spontaneous recovery.
- Histology and electron microscopy revealed various developmental stages of Wangiella dermatitidis, including sclerotic bodies, mycelial filaments, and yeast-like cells.
- The polymorphous nature of the fungus was evident throughout the infection phases.
Conclusions:
- Wangiella dermatitidis can establish experimental infections in the rabbit eye.
- The rabbit eye model shows potential for spontaneous resolution of fungal keratitis.
- Advanced microscopy is valuable for studying fungal morphology and host-pathogen interactions in ocular infections.