Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarization-dependent statistical models of sea clutter.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Effects of surface roughness of a waveplate on the development of diffractive polarization speckle.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Holographic tomographic volumetric additive manufacturing.

Nature communications·2025
Same author

Accelerated phase retrieval using adaptive support and statistical fringe processing of phase estimates.

Optics letters·2024
Same author

HoloTile light engine: new digital holographic modalities and applications.

Reports on progress in physics. Physical Society (Great Britain)·2024
Same author

Phase-only steerable photonic nanojets.

Optics express·2023
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Single-plane multiple speckle pattern phase retrieval using a deformable mirror.

Percival F Almoro1, Jesper Glückstad, Steen G Hanson

  • 1National Institute of Physics, University of the Philippines, Quezon City 1101, Philippines. pfalmoro@gmail.com

Optics Express
|October 14, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces an adaptive optics system using a deformable mirror (DM) for faster phase retrieval from multiple speckle patterns. The novel technique successfully reconstructs phase information even with non-Nyquist sampled data.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Related Experiment Videos

Last Updated: Jun 8, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Area of Science:

  • Optics and Photonics
  • Wavefront Sensing and Adaptive Optics

Background:

  • Phase retrieval is crucial for optical system characterization.
  • Conventional methods using multiple planes are time-consuming.
  • Adaptive optics (AO) offers potential for dynamic wavefront control.

Purpose of the Study:

  • To develop and analyze a single-plane phase retrieval technique using a deformable mirror (DM).
  • To replace time-consuming axial displacements with an adaptive optical system.
  • To evaluate the performance of the DM-based system for dynamic wavefronts.

Main Methods:

  • Analysis using complex ABCD-matrices for system design.
  • Implementation of a variable focal length DM at a Fourier plane.
  • Experimental evaluation using a conventional free-space phase retrieval setup.
  • Investigation of Nyquist and non-Nyquist sampling conditions.

Main Results:

  • The deformable mirror (DM) system replaces multiple axial measurements.
  • Pixelation and cross-talk issues of spatial light modulators are avoided.
  • Speckle patterns measured at the non-Nyquist range yield good phase reconstructions.
  • Successful reconstruction of spherical phase patterns with 50-micron fringe spacing demonstrated.

Conclusions:

  • The proposed AO system with a DM is effective for single-plane phase retrieval.
  • The technique is suitable for dynamic wavefronts and offers improved efficiency.
  • The DM-based method provides robust phase reconstruction even under non-ideal sampling conditions.