Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assessment of Diffusion and Perfusion01:17

Assessment of Diffusion and Perfusion

Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this principle...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lamin B1 safeguards the B cell genome and shapes lymphoma outcome.

HemaSphere·2026
Same author

Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.

Journal of translational medicine·2026
Same author

Cell aging - a relevant factor in live cell microscopy (mini-review).

Progress in biophysics and molecular biology·2025
Same author

<i>Pseudosulfitobacter pseudonitzschiae</i> hitchhikes on gliding colonies of <i>Cellulophaga lytica</i>.

ISME communications·2025
Same author

Perspective Article: Space-time dynamics of genome replication studied with super-resolved microscopy.

Postepy biochemii·2024
Same author

Modulated illumination microscopy: Application perspectives in nuclear nanostructure analysis.

Journal of microscopy·2024
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

Measuring and imaging diffusion with multiple scan speed image correlation spectroscopy.

Nadine Gröner1, Jérémie Capoulade, Christoph Cremer

  • 1Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany.

Optics Express
|October 14, 2010
PubMed
Summary
This summary is machine-generated.

Multiple scan speed image correlation spectroscopy (msICS) offers a new way to study how molecules move within cells. This method provides detailed mobility maps without needing prior knowledge of diffusion, making it versatile for biological research.

More Related Videos

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
07:05

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section

Published on: June 20, 2025

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Area of Science:

  • Cellular and Molecular Biology
  • Biophysics
  • Microscopy and Imaging Techniques

Background:

  • Intracellular mobility of biomolecules is vital for cellular functions, influenced by transport, diffusion, and molecular interactions.
  • Confocal laser scanning microscopy (CLSM) and Fluorescence Correlation Spectroscopy (FCS) are established methods for studying biomolecule dynamics.

Purpose of the Study:

  • To introduce and validate multiple scan speed image correlation spectroscopy (msICS) as an advanced technique for analyzing intracellular mobility.
  • To demonstrate msICS's capability to provide spatially resolved mobility information without requiring prior knowledge of diffusion properties.

Main Methods:

  • Development and application of multiple scan speed image correlation spectroscopy (msICS), an extension of raster image correlation spectroscopy (RICS).
  • Utilizing spatio-temporal information from CLSM scan processes to quantify molecular mobility.
  • Testing msICS with fluorophores in solution and free EGFP in living cells.

Main Results:

  • msICS successfully extracts spatially resolved mobility information using widely available CLSM instrumentation.
  • The method covers a broad dynamic range and generates correlation data comparable to FCS.
  • Two-dimensional maps of diffusion coefficients can be derived, demonstrating applicability in complex biological environments.

Conclusions:

  • msICS offers a powerful, versatile, and accessible alternative to existing methods for quantifying intracellular biomolecule mobility.
  • The technique's ability to map diffusion coefficients in situ enhances our understanding of cellular processes.
  • msICS expands the toolkit for biophysical characterization of molecular dynamics in living systems.