Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Imaging Fourier spectrometer in visible domain: design concept.

Applied optics·2020

Same author

Imaging Fourier spectrometer in visible domain: experimental results.

Applied optics·2020

Same author

Combined VIS-IR spectrometer with vertical probe beam.

The Review of scientific instruments·2018

Same author

Dual channel heterodyne microscope.

Journal of microscopy·2010

Same author

A branched pathway for peroxisomal protein import: S. cerevisiae ghosts and an intraperoxisomal PTS2 receptor.

Annals of the New York Academy of Sciences·1996

Same author

Targeting of human catalase to peroxisomes is dependent upon a novel C-terminal peroxisomal targeting sequence.

Annals of the New York Academy of Sciences·1996

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Dual-channel heterodyne microscope: new functions.

¹Moscow State University, Moscow, Russia. v.protopopov@hotmail.com

Journal of Microscopy

|October 16, 2010

Summary

A new super-resolution microscope offers advanced features for analyzing sub-wavelength structures and surfaces. Its enhanced modes, easily activated, demonstrate significant improvements in performance for diverse sample types.

Area of Science:

Optical Microscopy
Nanotechnology
Surface Science

Background:

Super-resolution microscopy is crucial for nanoscale imaging.
Heterodyne interferometry offers high sensitivity in optical measurements.
Characterization of sub-wavelength structures and anisotropic surfaces requires advanced techniques.

Purpose of the Study:

To introduce new features and performance capabilities of a super-resolution dual-channel heterodyne microscope.
To demonstrate the versatility of the microscope for quantitative assessment and profiling.
To showcase the ease of activating advanced operational modes.

Main Methods:

Utilized a super-resolution dual-channel heterodyne microscope.
Activated dark-field and bright-field modes via polarizer rotation or prism insertion.

More Related Videos

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Related Experiment Videos

Last Updated: Jun 8, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Performed quantitative assessment of sub-wavelength structures and 3D profiling of opaque/transparent samples.

Main Results:

Demonstrated recognition of nonpatterned anisotropic surfaces.
Achieved quantitative assessment of critical dimensions of sub-wavelength structures.
Successfully performed three-dimensional profiling of both opaque and transparent samples.

Conclusions:

The enhanced heterodyne microscope offers versatile new capabilities for nanoscale analysis.
Simple adjustments allow for activation of advanced imaging modes, expanding its utility.
The presented results highlight the microscope's improved performance and broad applicability.