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Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
Published on: September 18, 2018
A role for class 3 semaphorins in prostate cancer
V Blanc1, J Nariculam, P Munson
1Prostate Cancer Research Center, UCL London, UK.
The Prostate
|October 16, 2010
Summary
Class 3 semaphorins are key guidance cues in cell migration. In prostate cancer, Semaphorin 3E (Sema3E) is overexpressed, affecting cell adhesion and motility, suggesting it as a potential therapeutic target.
Area of Science:
- Cell biology
- Molecular oncology
- Cancer research
Background:
- Class 3 semaphorins are secreted proteins functioning as guidance cues for cell migration.
- They interact with transmembrane receptors, plexins and neuropilins.
- Semaphorins influence tumor progression directly and indirectly via angiogenesis.
Purpose of the Study:
- To investigate the role of semaphorins and their receptors in prostate cancer.
- To determine the effect of Semaphorin 3E (Sema3E) on prostate cancer cell behavior.
Main Methods:
- Quantitative reverse transcription PCR (RT-PCR), Western blotting, and immunohistochemistry were used to assess semaphorin and receptor expression.
- Adhesion and transwell migration assays were performed to evaluate Sema3E's impact on prostate cancer cell lines.
Main Results:
- Semaphorins, plexins, and neuropilins were co-expressed in prostate cancer cell lines and tissues.
- Sema3E was significantly overexpressed in tumor tissue and affected cancer cell adhesion to fibronectin and inhibited motility.
- Hypoxia modulated Sema3C, Sema3A, and Sema3E expression, suggesting anti-angiogenic roles for Sema3A and 3E.
Conclusions:
- Semaphorin 3E is aberrantly expressed in prostate cancer, influencing cancer cell adhesion and motility.
- The Sema3E/PlexinD1 signaling pathway plays a role in prostate cancer.
- Sema3E represents a potential therapeutic target for prostate cancer treatment.
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