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Updated: Jun 7, 2026

Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
Published on: October 6, 2022
Purification of plastocyanin and cytochrome c6 from plants, green algae, and cyanobacteria
José A Navarro1, Manuel Hervás, Miguel A De la Rosa
1Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla y Consejo Superior de Investigaciones Científicas, Sevilla, Spain.
Abstract:
Plastocyanin and cytochrome c6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. Such a difference in their electrostatic features is a critical factor in designing the purification procedure, which must thus be modified and adapted accordingly. This chapter reports the methods for producing (including cell cultures), isolating, and purifying plastocyanin and cytochrome c6--which greatly differ in their isoelectric point--from a number of eukaryotic and prokaryotic organisms.
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