[Echinocytosis and changes of medium weight molecules content in endo- and exogenous intoxications]

The purpose of this study was to detect the interrelationship between the increased serum concentration of the medium weight molecules (MWM) and echinocytosis, to establish the rate of echinocyte appearance in blood in endogenous and exogenous intoxications and the dependence of echinocytosis on phosphacol and carbophos doses or the degree of endogenous intoxication. Two series of studies were conducted. In the 1st series, the experiments were conducted on outbred albino rats. Rats of the 1st group received phosphacol in doses equal to 0.5, 5.0 and 50 LD50, while the rats of the 2nd group were given carbophos in doses equal to 0.1 and 1.0 LD50. In the 2nd series, the blood of cats with chronic renal failure at uremic stage was studied (as an example of endogenous intoxication). In all the animals, serum MWM concentrations were measured together with the relative echinocyte content and echinocytes were examined with electrone microscope. The ability of MWM to influence erythrocyte deformation was evaluated. Increased MWM concentrations and echinocyte content was found in association with the increase of intoxication severity, while echinocyte percentage in cats' blood was augmented under the action of MWM.