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Angular distribution of fluorescence from dye-filled capillaries.

B V Hunter, W S Bickel

|October 22, 2010

Summary

Fluorescence from liquid dye in capillaries is isotropic and unaffected by temperature, unlike solids. This finding optimizes viewing angles for capillary zone electrophoresis by minimizing laser light scattering.

Area of Science:

Optics and Photonics
Physical Chemistry
Analytical Chemistry

Background:

Studies on fluorescence from solid cylinders exist.
Theoretical expectations for solids differ from experimental observations.
Previous investigations reported conflicting results.

Purpose of the Study:

To investigate the angular distribution of fluorescence from a lossy capillary filled with laser dye.
To determine the temperature dependence of fluorescence isotropy in the liquid core.
To identify optimal viewing angles for capillary zone electrophoresis (CZE) experiments.

Main Methods:

Experimental study of fluorescence angular distribution.
Utilized a small, lossy capillary filled with laser-dye solution.

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Analysis of fluorescence in relation to temperature and proximity to phase transitions.

Main Results:

Fluorescence from the liquid core was found to be isotropic.
Isotropy showed no temperature dependence, even near phase transitions.
This behavior contrasts with theoretical predictions for solids and prior reports.

Conclusions:

Molecular motion in the liquid explains the observed isotropic fluorescence.
An optimal viewing angle near 90° is recommended for CZE.
This angle minimizes elastic scattering, reducing stray laser light contamination in optical systems.