Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ethical, legal, and social issues associated with human fetal tissue research in Japan.

Regenerative medicine·2026

Same author

Changing Discourse on In Vitro Gametogenesis: Expectation, Scientific Reality, and the Ethics of Hype.

Monash bioethics review·2026

Same author

Filamin-ETV4/5 acts as mechanosensor-mechanotransducer axis that drives cell competition-mediated elimination of transformed cells.

Nature communications·2026

Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026

Same author

Reconstitution of sex determination and the testicular niche using mouse pluripotent stem cells.

Science (New York, N.Y.)·2026

Same author

Evolutionarily divergent transcriptomic programs in ovarian folliculogenesis across mice, monkeys and humans.

Development (Cambridge, England)·2026

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

A global single-cell cDNA amplification method for quantitative microarray analysis.

Kazuki Kurimoto¹, Mitinori Saitou

¹Laboratory for Mammalian Germ Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan. kurimoto@cdb.riken.jp

Methods in Molecular Biology (Clifton, N.J.)

|October 23, 2010

Summary

This summary is machine-generated.

This study presents a new protocol for amplifying complementary DNAs (cDNAs) from single cells, preserving their orientation for microarray analysis. This method enables accurate gene expression profiling from limited biological samples.

More Related Videos

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

Related Experiment Videos

Last Updated: Jun 7, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Accurate gene expression analysis requires reliable amplification of complementary DNAs (cDNAs).
Single-cell analysis presents challenges due to limited starting material.
Existing methods may not preserve crucial information like sense-antisense orientation.

Purpose of the Study:

To develop a robust protocol for amplifying global cDNAs from individual cells.
To ensure the amplified cDNAs retain their sense-antisense orientation.
To facilitate template preparation for quantitative high-density oligonucleotide microarray analyses.

Main Methods:

Single-cell lysis followed by sequential cDNA synthesis steps.
Inclusion of oligo dT-tailed primers for first and second-strand synthesis.
Poly (dA) tailing and directional PCR amplification.
T7 RNA polymerase promoter addition via PCR for cRNA synthesis and microarray hybridization.

Main Results:

Successful amplification of global cDNAs from single cells.
Preservation of sense-antisense orientation in amplified cDNAs.
Demonstrated applicability for quantitative high-density oligonucleotide microarray analyses.

Conclusions:

The described protocol provides faithful amplification of single-cell cDNAs.
The method preserves essential orientation information for downstream applications.
This technique is valuable for gene expression profiling using microarrays with limited cell input.