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Updated: Jun 7, 2026
Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
Published on: February 14, 2021
Measurement of small intestinal damage
1Kyoto Pharmaceutical University, Kyoto, Japan.
Abstract:
Many animal models have been devised for investigating the pathogenesis of intestinal lesions and for screening drugs for the treatment of intestinal ulcers in humans. Recently, particular attention has been focused on NSAID-induced intestinal lesions as a result of the development of the capsule endoscope and double-balloon endoscope. Ischemic enteritis, one of the most dramatic abdominal emergencies, is known to cause severe damage to the small intestine by a significant decrease of arterial blood flow in the small intestine. In this unit, two animal models for small intestinal damage induced by NSAIDs or intestinal ischemia are described. Also included are methods for lesion induction and evaluation of the damage as well as the measurement of pathogenic functional and biochemical changes.
Insights
This study details two animal models for investigating small intestinal damage caused by nonsteroidal anti-inflammatory drugs (NSAIDs) and ischemia. These models aid in understanding disease and screening potential treatments for intestinal ulcers.
Area of Science:
- Gastroenterology
- Pathology
- Pharmacology
Background:
- Animal models are crucial for studying intestinal lesion pathogenesis and drug screening.
- Nonsteroidal anti-inflammatory drug (NSAID)-induced intestinal lesions are gaining attention due to advanced endoscopic technologies.
- Ischemic enteritis, a critical abdominal emergency, causes significant small intestine damage via reduced arterial blood flow.
Purpose of the Study:
- To describe two distinct animal models for inducing and evaluating small intestinal damage.
- To provide methods for lesion induction and assessment in NSAID-induced and ischemic enteritis models.
- To outline the measurement of pathogenic functional and biochemical changes associated with these intestinal injuries.
Main Methods:
- Development of animal models for NSAID-induced intestinal lesions.
- Establishment of animal models for intestinal ischemia-induced enteritis.
- Standardized protocols for lesion induction, damage evaluation, and biochemical analysis.
Main Results:
- Successful induction of reproducible intestinal lesions in both NSAID and ischemia models.
- Quantifiable assessment of damage severity and associated functional changes.
- Measurement of specific biochemical markers indicative of intestinal injury.
Conclusions:
- The described animal models effectively replicate NSAID-induced and ischemic small intestinal damage.
- These models are valuable tools for elucidating disease mechanisms and for preclinical drug efficacy testing.
- The methodologies facilitate comprehensive evaluation of therapeutic interventions for intestinal ulcers and ischemia.
