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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Fast, high resolution mass spectrometry imaging using a Medipix pixelated detector
Julia H Jungmann1, Luke MacAleese, Ronald Buijs
1FOM Institute for Atomic and Molecular Physics (AMOLF), Amsterdam, The Netherlands.
Journal of the American Society for Mass Spectrometry
|October 26, 2010
Summary
A new position-sensitive detector enhances ion microscope mass spectrometry imaging, achieving sub-6 μm spatial resolution for detailed sample analysis.
Area of Science:
- Analytical Chemistry
- Materials Science
- Spectroscopy
Background:
- Mass spectrometry imaging (MSI) pushes spatial resolution limits.
- Ion microscopes magnify ion distributions from samples onto detectors.
- Time-of-flight mass separation is crucial for MSI.
Purpose of the Study:
- To introduce a novel position-sensitive detector for ion microscope MSI.
- To evaluate the performance of this new detector system.
- To demonstrate improved spatial resolving power in MSI.
Main Methods:
- Coupling a chevron microchannel plate stack and a 512 × 512 CMOS pixel detector to an ion microscope.
- Utilizing secondary ion mass spectrometry (SIMS) for analysis.
- Employing laser desorption ionization (LDI) for analysis.
Main Results:
- Achieved spatial resolving power better than 6 μm using SIMS.
- Demonstrated 8-10 μm spatial resolving power with LDI.
- Presented a detailed evaluation of spatial resolution, acquisition speed, and data handling.
Conclusions:
- The new detector system significantly enhances spatial resolution in ion microscope MSI.
- This advancement enables more detailed analysis of sample surfaces.
- The system offers a robust platform for various MSI applications.
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