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Updated: Jun 7, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Isobaric protein and peptide quantification: perspectives and issues.

Achim Treumann¹, Bernd Thiede

¹NEPAF, Devonshire Building, Newcastle upon Tyne, NE1 7RU, UK.

Expert Review of Proteomics

|October 27, 2010

Summary

This summary is machine-generated.

Comparing protein levels across samples is key in proteomics. Isobaric peptide labeling techniques like iTRAQ and tandem mass tagging reduce sample complexity for accurate relative quantification.

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Last Updated: Jun 7, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

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Published on: August 17, 2015

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Proteomics
Analytical Chemistry

Background:

Comparing protein levels across biological samples is a significant challenge in proteomics.
Isotopic and isobaric peptide labeling are crucial for relative proteome quantification.

Purpose of the Study:

To address the increased sample complexity associated with traditional isotopic labeling methods.
To highlight the advantages of isobaric labeling strategies in quantitative proteomics.

Main Methods:

Review of established isobaric labeling strategies including isobaric tag for relative and absolute quantification (iTRAQ), tandem mass tagging, cleavable isobaric affinity tag, dimethylated leucines, and isobaric peptide termini labeling.
Discussion of multiplexing applications for iTRAQ and tandem mass tagging.

Main Results:

Isobaric labeling strategies effectively mitigate the sample complexity issue inherent in many isotopic labeling techniques.
These advanced methods enable more precise and efficient relative protein quantification.

Conclusions:

Isobaric labeling techniques represent a significant advancement in quantitative proteomics.
The development of strategies like iTRAQ and tandem mass tagging has expanded the capabilities for multiplexed proteome analysis.