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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis01:22

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...

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Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Male gametogenesis without centrioles.

Maria Giovanna Riparbelli1, Giuliano Callaini

  • 1University of Siena, Department of Evolutionary Biology, Via A. Moro 2, I-53100 Siena, Italy.

Developmental Biology
|October 27, 2010
PubMed
Summary

Centrioles are not essential for mitosis in Drosophila male germline stem cells, but are crucial for proper meiotic spindle assembly during gametogenesis. This highlights distinct centriole roles in cell division.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Mitotic spindle orientation is crucial for stem cell renewal and differentiation.
  • Asymmetric cell division relies on precise spindle positioning.
  • Centrioles are key components of the centrosome, involved in spindle formation.

Purpose of the Study:

  • To investigate the role of centrioles in male gametogenesis in Drosophila.
  • To determine if centrioles are essential for mitotic spindle function in male germline stem cells.
  • To analyze the impact of centriole absence on meiotic spindle assembly.

Main Methods:

  • Analysis of DSas-4 Drosophila mutants lacking centrioles.
  • Microscopic examination of male germline stem cell divisions (mitosis).

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

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Manipulation of Ploidy in Caenorhabditis elegans
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Manipulation of Ploidy in Caenorhabditis elegans

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Related Experiment Videos

Last Updated: Jun 7, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Manipulation of Ploidy in Caenorhabditis elegans
07:54

Manipulation of Ploidy in Caenorhabditis elegans

Published on: March 15, 2018

  • Observation of meiotic spindle formation in primary spermatocytes.

    • Main Results:

    • Spindle alignment and asymmetric divisions occur normally in centriole-lacking male germline stem cells.
    • Spermatogonial divisions proceed correctly, forming 16-cell cysts.
    • Abnormal meiotic spindles form in primary spermatocytes lacking centrioles.

    Conclusions:

    • Centrioles are dispensable for mitotic spindle formation in Drosophila male germline stem cells.
    • Centrioles are essential for proper meiotic spindle assembly during male gametogenesis.
    • Drosophila male gametogenesis exhibits differential requirements for centrioles in mitosis versus meiosis.