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Published on: February 4, 2021
HeLa cell transfection using a novel sonoporation system.
Somphop Rodamporn1, N R Harris, Steve P Beeby
1School of Electronics and Computer Science, University of Southampton, Southampton, SO171BJ, UK. somphop@swu.ac.th
IEEE Transactions on Bio-Medical Engineering
|October 28, 2010
Summary
This study introduces a novel microfluidic sonoporation system that enhances gene therapy and drug delivery. The resonant system achieves high cell transfection efficiency and viability using ultrasonic standing waves.
Area of Science:
- Biotechnology and Biomedical Engineering
- Cellular and Molecular Biology
Background:
- Sonoporation is crucial for gene therapy and drug delivery.
- Existing methods face challenges in efficiency and cell viability.
Purpose of the Study:
- To develop and validate a novel microfluidic sonoporation system.
- To optimize cell transfection and viability using resonant frequencies.
Main Methods:
- Theoretical analysis of a resonant sonoporation chamber design.
- Utilized a piezoelectric transducer (PZT 26) to generate ultrasound at 980 kHz.
- Experimental validation using HeLa cells and plasmid (peGFP-N1) under various conditions.
Main Results:
- Achieved 68.9% cell transfection efficiency at 980 kHz and 100 V(p-p).
- Maintained 77% cell viability after 10 seconds of sonoporation.
- Demonstrated significant results with ANOVA (p < 0.05).
Conclusions:
- The microfluidic resonant sonoporation system significantly improves transfection efficiency and cell viability.
- This technology holds promise for advanced gene therapy and drug delivery applications.
