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Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Jun 7, 2026

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

FAIR: A server for internal sequence repeats.

Ramaswamy Senthilkumar1, Radhakrishnan Sabarinathan, Bazil Shaahul Hameed

  • 1Bioinformatics Centre, Centre of Excellence in Structural Biology and Bio-computing, Indian Institute of Science, Bangalore 560 012, India.

Bioinformation
|October 28, 2010
PubMed
Summary
This summary is machine-generated.

A new internet server identifies internal sequence repeats in protein and DNA sequences. Users can also search for these repeats in other sequence and structure databases, accessing the latest information.

Keywords:
DNAinternal repeatsproteinsequenceserver

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Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Internal sequence repeats are crucial in protein and DNA sequences.
  • Identifying these repeats aids in understanding sequence function and evolution.
  • Existing methods may lack comprehensive search capabilities across multiple databases.

Purpose of the Study:

  • To develop an internet computing server for identifying internal sequence repeats in protein and DNA.
  • To enable users to search for identified repeats in external sequence and structure databases.
  • To provide access to up-to-date information from integrated databases.

Main Methods:

  • Development of an internet computing server.
  • Implementation of algorithms to detect internal sequence repeats in biological sequences.
  • Integration with up-to-date protein and DNA sequence databases.
  • Inclusion of functionality to query the Protein Data Bank (PDB).

Main Results:

  • Successful identification of internal sequence repeats in protein and DNA.
  • Capability to cross-reference identified repeats with other sequence and structure databases.
  • Provision of the latest available data due to updated databases.

Conclusions:

  • The developed server offers a comprehensive tool for analyzing internal sequence repeats.
  • It facilitates comparative analysis across different biological sequence and structure datasets.
  • The freely accessible web server enhances research accessibility in bioinformatics.