Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosensors for directly measuring cell affecting agents.

J W Parce1, J C Owicki, K M Kercso

  • 1Molecular Devices Corp., Menlo Park, CA 94025.

Annales De Biologie Clinique
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Testing ocular irritancy in vitro with the silicon microphysiometer.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

Multiport flow-control system for lab-on-a-chip microfluidic devices.

Fresenius' journal of analytical chemistry·2001
Same author

Synchronized cyclic capillary electrophoresis using channels arranged in a triangle and low voltages.

Fresenius' journal of analytical chemistry·2001
Same author

Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer.

Journal of biomolecular screening·2000
Same author

Electrokinetically controlled microfluidic analysis systems.

Annual review of biophysics and biomolecular structure·2000
Same author

Test tube's end.

Journal of biomolecular screening·2000
Same journal

A morphology-driven proof-of-concept study linking interphase nuclear abnormalities to dicentric-mediated genomic instability.

Annales de biologie clinique·2026
Same journal

Analytical validation and clinical concordance of Diazyme free light chain assays on the Cobas® platform compared with Freelite™.

Annales de biologie clinique·2026
Same journal

Small-cell secondary plasma cell leukemia mimicking low-grade B-cell lymphoma.

Annales de biologie clinique·2026
Same journal

[Multiple myeloma and analytical discrepancy in serum free light chain assay: a case report].

Annales de biologie clinique·2026
Same journal

[Summary of the 34th National Conference of the National College of Hospital Biochemistry].

Annales de biologie clinique·2026
Same journal

Assessment of the carbon footprint of an automated biochemistry toxicology platform: feedback from Rouen University Hospital.

Annales de biologie clinique·2026
See all related articles

New cellular perfusion chambers utilizing Light Addressable Potentiometric Sensors (LAPS) enable rapid measurement of cell metabolic rates. This technology quickly assesses cellular responses to agents, aiding drug development and toxicity testing.

Area of Science:

  • Biomedical Engineering
  • Cellular Biology
  • Sensor Technology

Background:

  • Cellular metabolic rate is a key indicator of cellular function and response to external stimuli.
  • Accurate and rapid measurement of metabolic activity is crucial for drug discovery and toxicology.
  • Existing methods for measuring cellular metabolism can be time-consuming or require complex sample handling.

Purpose of the Study:

  • To develop and validate novel cellular perfusion chambers using Light Addressable Potentiometric Sensor (LAPS) technology.
  • To assess the utility of these chambers for measuring cellular metabolic rates in response to various agents.
  • To demonstrate the application of this system in evaluating receptor activation, compound toxicity, and chemotherapeutic efficacy.

Main Methods:

Related Experiment Videos

  • Construction of cellular perfusion chambers integrated with a Light Addressable Potentiometric Sensor (LAPS).
  • Utilized a stopped-flow mode for sample introduction and metabolic rate determination.
  • Measured metabolic rates of various mammalian cell types, including normal human cells and multidrug-resistant tumor cells.

    • Main Results:

    • Demonstrated rapid (minutes) determination of cellular receptor triggering.
    • Correlated metabolic inhibition of normal human cells with in vivo ocular irritancy of test compounds.
    • Successfully determined the efficacy of chemotherapeutic agents against multidrug-resistant tumor cells within hours.

    Conclusions:

    • Cellular perfusion chambers based on LAPS technology provide a rapid and sensitive platform for assessing cellular metabolic activity.
    • This system offers a valuable tool for high-throughput screening of drug candidates, toxicity assessment, and evaluating cancer therapeutic responses.