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Updated: Aug 4, 2026
11:22
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
Total chemical assembly of genes
Z A Shabarova1, N I Sokolova, I N Merenkova
1Chemistry Department, Moscow State University, USSR.
Nucleic Acids Symposium Series
|January 1, 1990
Abstract:
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribonucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degree C in buffer. The resulting gene was cloned into M13mp11 and sequenced using the Sanger sequencing procedure.
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