Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Published on: June 21, 2012

Methods for culturing mouse and human embryonic stem cells.

Sabrina Lin¹, Prue Talbot

¹Department of Cell Biology & Neuroscience and Stem Cell Center, University of California Riverside, Riverside, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 3, 2010

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Methylglyoxal and Glyoxal in High-Puff Disposable Electronic Cigarette Liquids: Unexpected Accumulation and Enhanced Cytotoxicity.

ACS omega·2026

Same author

SARS-CoV-2 pseudoparticles preferentially infect ectoderm in human embryonic tissues.

Frontiers in cell and developmental biology·2026

Same author

Focal Transcranial Magnetic Stimulation of the Rat Anterior Cingulate Cortex Inhibits Incubation of Opioid Craving after Voluntary Abstinence.

bioRxiv : the preprint server for biology·2026

Same author

Unraveling the Toxicological Effects of Hydroxyacetone─A Reaction Product in Electronic Cigarette Aerosols.

Chemical research in toxicology·2026

Same author

Acetaldehyde and methylglyoxal: comparative analysis of toxic electronic cigarette degradation products in 3D and 2D exposure systems using human bronchial epithelial models.

Frontiers in toxicology·2025

Same author

Addressing a common complication after ileostomy creation: a multidisciplinary program to decrease readmission for dehydration.

Clinics in surgery·2025

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

This study provides essential protocols for culturing mouse embryonic stem cells (mESCs) and human embryonic stem cells (hESCs). These methods support stem cell research, including the use of feeder layers and defined media for improved cell culture.

Area of Science:

Developmental Biology
Stem Cell Biology
Cell Culture Technology

Background:

Mouse embryonic stem cells (mESCs) have been vital for genetic studies and developmental research for decades.
Human embryonic stem cells (hESCs), derived in 1998, hold promise for regenerative medicine and toxicology.
Fibroblast feeder layers, often mouse embryonic fibroblasts (mEFs), are commonly used to support ESC culture, maintaining pluripotency and promoting growth.

Purpose of the Study:

To provide foundational protocols for isolating mouse embryonic fibroblasts (mEFs) and establishing feeder layers.
To detail methods for culturing mESCs on mEFs and gelatin in serum-containing media.
To outline protocols for culturing hESCs in defined media on mEFs and Matrigel.

Main Methods:

More Related Videos

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Published on: April 22, 2017

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

Published on: June 4, 2008

Related Experiment Videos

Last Updated: Jun 7, 2026

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Published on: June 21, 2012

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Published on: April 22, 2017

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

Published on: June 4, 2008

Isolation and culture of mEFs for feeder layer preparation.

Culture of mESCs using serum-containing media on feeder layers or gelatin.

Culture of hESCs in defined media on mEF feeder layers or Matrigel.

Main Results:

Established protocols for mESC and hESC culture were successfully tested.
Demonstrated methods for feeder layer preparation and utilization.
Provided options for both serum-containing and defined media culture conditions.

Conclusions:

The provided protocols are suitable for researchers establishing stem cell laboratories.
These methods offer a basis for further adaptation and modification for specific research needs.
The study facilitates the advancement of stem cell research in both mouse and human models.