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Updated: Jun 7, 2026
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
[Study on the growth of H1299 cell line transfected transiently with mouse p53 minigenes with different codon 172
Background:
To investigate the effect on cell growth after transfected with mouse 172 wild-type p53 (Arg), pseudo-wild-type p53 (Arg->Leu) and mutant p53 (Arg->His).
Methods:
Vectors of three kinds of p53 minigene were transfected into p53-null H1299 cells through lipofectAMINE. Cells were collected at different time after transfection and analyzed by cell counting, flow cytometry and Western blotting.
Results:
Wild-type p53 and pseudo-wild-type p53 could induce cell apoptosis and cell cycle arrest at different time. Bax, p21(WAF1) and Mdm-2 were transactivated by wild-type p53 and pseudo-wild-type p53. No tumor growth suppression was observed in mutant p53 transfected cells.
Conclusions:
Wild-type p53 and pseudo-wild-type p53 can inhibit cell growth by inducing apoptosis and cell cycle arrest through transactivation of Bax and p21(WAF1). There exists feedback loop between Mdm-2 and p53. The different biological effect between 172Leu and 172His shows that some p53 variants such as 172 Arg->Leu may act as wild-type p53.
