Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

Gas Chromatography: Types of Detectors-I

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Trianalyte µPAD for Simultaneous Determination of Iron, Zinc, and Manganese Ions.

Molecules (Basel, Switzerland)·2024

Same author

Hydrogen peroxide stabilization with silica xerogel for paper-based analytical devices and its application to phenolic compounds determination.

Analytica chimica acta·2024

Same author

Multipoint monitor of beer fermentation.

Food chemistry·2024

Same author

Microfluidic paper-based analytical device for simultaneous determination of calcium and magnesium ions in human serum.

Analytica chimica acta·2024

Same author

Turbidimetric flow analysis system for the investigation of microbial growth.

Talanta·2023

Same author

Photometric flow system for the determination of serum lactate dehydrogenase activity.

Talanta·2023

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

Hemoglobin determination with paired emitter detector diode.

Elżbieta Mieczkowska¹, Robert Koncki, Łukasz Tymecki

¹University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.

Analytical and Bioanalytical Chemistry

|November 4, 2010

Summary

A low-cost photometric hemoglobinometer was developed using green light-emitting diodes. This device accurately measures total hemoglobin (Hb) in diluted blood samples, showing results compatible with clinical analyzers.

More Related Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Related Experiment Videos

Last Updated: Jun 7, 2026

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Area of Science:

Biomedical Engineering
Clinical Chemistry

Background:

Accurate hemoglobin measurement is crucial for diagnosing and monitoring various medical conditions.
Existing clinical analyzers for hemoglobin determination can be expensive and complex.

Purpose of the Study:

To develop a cost-effective photometric hemoglobinometer prototype for total hemoglobin (Hb) analysis.
To optimize and validate the device for clinical applications using standard chemical methods.

Main Methods:

Utilized two ordinary green light-emitting diodes as emitter and detector, coupled with a simple voltmeter.
Optimized cuvette assays for diluted blood samples using Drabkin, lauryl sulfate, and dithionite chemical methods.
Validated the prototype's performance by assaying total Hb content in human blood.

Main Results:

The developed device functions as a complete, cost-effective photometric hemoglobinometer.
Assays using the prototype demonstrated full compatibility with results from clinically recommended methods and a clinical analyzer.
The device proved effective for real-world analytical applications.

Conclusions:

A simple, inexpensive photometric device using light-emitting diodes can accurately quantify total hemoglobin.
This prototype offers a viable alternative for hemoglobin testing, particularly in resource-limited settings.
The developed hemoglobinometer shows promise for routine clinical analysis and diagnostics.