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Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex

Youngseon Choi1, Junghan Lee, Keumhyun Kim

  • 1Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 696 Sampyeong-dong, Bundang-gu, Seongnam-Si, Gyeonggi-Do, 464-400, South Korea.

Chemical Communications (Cambridge, England)
|November 5, 2010
PubMed

Keywords:
fluorescence resonance energy transferlive cell imagingprotease inhibitor screeningnanosensor development

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...