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Related Experiment Video

Updated: Jun 7, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Published on: May 5, 2014

A high-throughput screening strategy to overcome virus instability.

Lynn Rasmussen1, Clinton Maddox, Blake P Moore

  • 1High Throughput Screening Center, Southern Research Institute, Birmingham, Alabama 35205, USA. rasmussen@sri.org

Assay and Drug Development Technologies
|November 6, 2010
PubMed
Summary

Researchers developed a stable, high-throughput screening assay for respiratory syncytial virus (RSV) drug discovery. This new cell-based assay overcomes virus instability, enabling efficient screening of compounds to find new antiviral treatments.

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Area of Science:

  • Virology
  • Infectious Diseases
  • Drug Discovery

Background:

  • Respiratory syncytial virus (RSV) causes severe illness in vulnerable populations.
  • RSV vaccine and drug development are hindered by virus instability.
  • Existing antiviral assays lack robustness for high-throughput screening.

Purpose of the Study:

  • To develop and validate a stable, cell-based high-throughput screening assay for RSV inhibitors.
  • To overcome the challenge of virus instability in RSV research.
  • To facilitate efficient drug discovery for RSV.

Main Methods:

  • Utilized frozen RSV-infected cells as a source of infectious material.
  • Developed a cell-based assay to screen for inhibitors of RSV-induced cytopathic effect.

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  • Validated the assay using 1,280 compounds previously identified as potentially active against RSV.

    • Main Results:

    • Successfully overcame the problem of virus instability using infected cells.
    • Validated a robust cell-based high-throughput screening assay for RSV.
    • Screened over 300,000 compounds with minimal variability in controls over several months.

    Conclusions:

    • The developed cell-based assay is stable and suitable for high-throughput screening.
    • This assay provides a viable platform for identifying novel RSV antiviral drugs.
    • The method addresses a critical limitation in RSV drug discovery research.