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Periodontal tissue engineering after tooth replantation
Luciana Reichert da Silva Assunção1, Renato Colenci, Caril Constante Ferreira do-Amaral
1School of Dentistry, Federal University of Pará, UFPA, Pará, Brazil. lurassuncao@yahoo.com.br
Journal of Periodontology
|November 9, 2010
Summary
Platelet-rich plasma (PRP) activated with thrombin shows promise for enhancing tooth replantation healing. However, PRP with calcium chloride alone was not an effective scaffold for undifferentiated cells in this study.
Area of Science:
- Oral surgery and regenerative medicine
- Biomaterials science
- Cell biology
Background:
- Blood-derived products like platelet-poor plasma (PPP) and platelet-rich plasma (PRP) are used to enhance tissue healing.
- PRP also serves as a scaffold for bone marrow stem cells in tissue engineering.
- This study investigates the efficacy of various PRP formulations in promoting the healing of replanted dog teeth.
Purpose of the Study:
- To evaluate the effects of PPP, calcium chloride-activated PRP (PRP/Ca), thrombin- and calcium chloride-activated PRP (PRP/Thr/Ca), and bone marrow mononuclear cells with PRP/Ca (BMMCs/PRP/Ca) on the healing of replanted dog teeth.
- To assess the potential of these blood-derived products as scaffolds for cell-based therapies in dental applications.
Main Methods:
- Teeth were extracted and replanted with control, PPP, PRP/Ca, PRP/Thr/Ca, or BMMCs/PRP/Ca.
- Histologic, histomorphometric, and immunohistochemical analyses were performed 120 days post-replantation.
- Statistical analysis included ANOVA, Tukey, Kruskal-Wallis, and Dunn tests.
Main Results:
- BMMCs/PRP/Ca demonstrated the most significant replacement resorption with osseous ingrowth and tartrate-resistant acid phosphatase (TRAP) activity.
- PRP/Ca showed replacement resorption with osteopontin expression.
- PRP/Thr/Ca did not exhibit replacement resorption, while PPP induced inflammatory resorption with TRAP activity.
Conclusions:
- Platelets activated with thrombin appear crucial for successful tissue healing following tooth replantation.
- PRP/Ca alone may not be a suitable scaffold for undifferentiated cells in treating avulsed teeth.
- Further research is needed to explore alternative materials for enhanced outcomes in tooth replantation.
