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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 6, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

CdSe quantum dots for two-photon fluorescence thermal imaging.

Laura Martinez Maestro¹, Emma Martín Rodríguez, Francisco Sanz Rodríguez

¹Fluorescence Imaging Group, Departamento de Física de Materiales, C-IV, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, Madrid 28049, Spain.

|November 11, 2010

Summary

This summary is machine-generated.

Cadmium selenide (CdSe) quantum dots function as effective fluorescent nanothermometers for two-photon microscopy. Their enhanced temperature sensitivity under near-infrared excitation allows for precise thermal imaging in biological samples.

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Published on: October 13, 2017

Area of Science:

Biomedical Engineering
Nanotechnology
Optical Imaging

Background:

Quantum dots (QDs) have revolutionized fluorescence bioimaging.
Multiphoton fluorescence microscopy offers enhanced spatial resolution.
Temperature sensitivity of QD fluorescence is crucial for bio-applications.

Purpose of the Study:

To evaluate Cadmium Selenide (CdSe) quantum dots as fluorescent nanothermometers.
To investigate their utility in two-photon fluorescence microscopy for thermal imaging.
To assess temperature sensitivity under one-photon versus two-photon excitation.

Main Methods:

Utilized CdSe quantum dots for fluorescence measurements.
Employed two-photon (near-infrared) and one-photon (visible) excitation microscopy.
Measured temperature sensitivity of emission intensity and peak wavelength.
Demonstrated thermal imaging in phosphate-buffered saline and single living cells.

Main Results:

Two-photon excitation exhibited significantly higher temperature sensitivity compared to one-photon excitation.
Peak emission wavelength showed temperature sensitivity, enabling thermal imaging of non-homogeneously dispersed nanoparticles.
Successfully imaged artificial temperature gradients and measured intracellular temperature increases in living cells.

Conclusions:

CdSe quantum dots are promising fluorescent nanothermometers for two-photon microscopy.
Superior thermal sensitivity under two-photon excitation enables advanced thermal imaging.
This technology facilitates precise temperature measurements in biological environments.