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Published on: January 25, 2018
Establishment and evaluation of event-specific quantitative PCR method for genetically modified soybean MON89788
Reona Takabatake1, Mari Onishi, Tomohiro Koiwa
1Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.
Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
|November 13, 2010
Summary
A new real-time PCR method accurately quantifies the genetically modified soybean event MON89788. This validated method is suitable for practical GMO detection and quantification, with a limit of 0.1%.
Area of Science:
- Agricultural Biotechnology
- Molecular Biology
- Food Science
Background:
- Accurate quantification of genetically modified organisms (GMOs) is crucial for regulatory compliance and consumer information.
- Specific quantification of novel GM soybean events like MON89788 requires validated analytical methods.
Purpose of the Study:
- To establish and validate a real-time PCR-based method for event-specific quantification of GM soybean event MON89788.
- To determine the conversion factor (Cf) necessary for accurate GMO amount calculation.
- To assess the method's performance through single-laboratory and multi-laboratory trials.
Main Methods:
- Development of a novel real-time PCR assay targeting the specific genetic modification in soybean event MON89788.
- Experimental determination of the conversion factor (Cf) for quantitative analysis.
- Validation of the method via single-laboratory analysis and a blind multi-laboratory trial.
Main Results:
- The real-time PCR method achieved event-specific quantification of GM soybean MON89788.
- The limit of quantitation was determined to be 0.1% or lower.
- Method performance metrics, including bias and reproducibility (RSD(R)), were less than 20%, indicating good trueness and precision.
Conclusions:
- The established real-time PCR method is suitable for the practical detection and quantification of GM soybean event MON89788.
- The method demonstrates reliable performance suitable for routine use in GMO analysis.
- Validation through multi-laboratory trials confirms the robustness of the developed quantification technique.
