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Updated: Jun 6, 2026
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
Adipogenic differentiation of adipose-derived stem cells
Gang Yu1, Z Elizabeth Floyd, Xiying Wu
1Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2010
Summary
Adipose-derived stem cells (ASCs) can be isolated and grown in labs. These cells can be stimulated in vitro to become fat cells, a process called adipogenesis.
Area of Science:
- Stem Cell Biology
- Tissue Engineering
- Cellular Differentiation
Background:
- Adipose-derived stem cells (ASCs) are multipotent stromal cells found in adipose tissue.
- ASCs possess the capacity for differentiation into various cell types, including adipocytes.
- The isolation, expansion, and cryopreservation of ASCs from diverse animal species are established procedures.
Purpose of the Study:
- To describe established methods for inducing adipogenesis in ASCs in vitro.
- To outline techniques for quantifying the extent of adipogenic differentiation.
- To provide a comprehensive guide for researchers working with ASCs and adipogenesis.
Main Methods:
- Isolation and expansion of ASCs from adipose tissue.
- Induction of adipogenesis using specific chemical cocktails or growth factors.
- Quantification of adipogenic differentiation through biochemical and morphological assays.
Main Results:
- Demonstration of successful in vitro adipogenic differentiation of ASCs.
- Validation of methods for measuring key adipogenic markers.
- Establishment of a reproducible protocol for ASC adipogenesis.
Conclusions:
- In vitro adipogenesis of ASCs is a feasible and quantifiable process.
- Standardized methods are crucial for reliable research in ASC differentiation.
- This chapter provides essential protocols for studying ASCs in regenerative medicine and research.
