Preservation protocols for human adipose tissue-derived adult stem cells
Ram Devireddy1, Sreedhar Thirumala
1Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA. devireddy@me.lsu.edu
Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2010
Summary
Simple storage methods for adult stem cells, including freezing and drying, are crucial for tissue engineering. Our lab developed optimal protocols for long-term adult stem cell preservation using these techniques.
Area of Science:
- Regenerative Medicine
- Biotechnology
- Cell Biology
Background:
- Effective storage of adult stem cells is vital for advancing tissue engineering.
- Current methods like cryopreservation, anhydrobiosis, and lyophilization have limitations.
- Cryopreservation requires complex procedures and cryoprotective agents (CPAs).
Purpose of the Study:
- To provide an overview of adult stem cell storage techniques.
- To present optimal protocols for long-term storage developed in our laboratory.
- To compare freezing and drying methods for stem cell preservation.
Main Methods:
- Review of established stem cell storage techniques: cryopreservation, anhydrobiosis, and lyophilization.
- Development and optimization of protocols for freezing storage.
- Development and optimization of protocols for drying storage.
Main Results:
- Cryopreservation involves complex steps and requires specific equipment and cryoprotective agents (CPAs).
- Drying methods, including anhydrobiosis, may require high CPA concentrations and disaccharides.
- Lyophilization combines cryopreservation with subsequent desiccation via sublimation.
- Optimal protocols for long-term storage using freezing and drying were established in the laboratory.
Conclusions:
- Simple and effective storage protocols are essential for enhancing the utility of adult stem cells in tissue engineering.
- Both freezing and drying storage methods offer viable options for long-term adult stem cell preservation.
- Further research into optimizing these protocols can improve stem cell applications.
