A novel embryo identification system by direct tagging of mouse embryos using silicon-based barcodes

Area of Science:

• Reproductive biology
• Biotechnology
• Medical device development

Background:

• Current assisted reproductive technologies (ART) protocols rely on labware labeling and double-witnessing, which do not fully eliminate identification error risks.
• Gamete and embryo handling during ART cycles involves multiple transfers, increasing the potential for mix-ups.
• Existing methods lack a robust system for direct embryo identification, necessitating novel solutions.

Purpose of the Study:

• To provide proof of concept for a direct embryo labeling system using silicon-based barcodes.
• To assess the feasibility of microinjecting silicon barcodes into the perivitelline space of embryos.
• To evaluate the impact of barcode labeling on embryo development, identification, and cryopreservation.

Main Methods:

• Three types of silicon-based barcodes were designed and microinjected into the perivitelline space of mouse pronuclear embryos.
• Embryos were cultured to the blastocyst stage, with assessments of development, barcode retention, and identification rates.
• Barcode release after hatching and system validity after cryopreservation were also evaluated.

Main Results:

• Silicon barcodes did not affect embryo development rates at any stage.
• Over 90% of embryos retained at least one barcode until the blastocyst stage.
• Using four type C barcodes achieved 97% embryo identification accuracy, unaffected by cryopreservation.

Conclusions:

• A direct embryo labeling system using silicon-based barcodes is feasible.
• This technology represents a significant starting point for developing improved ART identification methods.
• Silicon barcodes offer a promising solution to enhance accuracy and prevent mix-ups in fertility treatments.