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Updated: Jun 6, 2026

Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections
Published on: October 31, 2017
Novel gratisin derivatives with high antimicrobial activity and low hemolytic activity
Makoto Tamaki1, Yukie Imazeki, Aya Shirane
1Department of Chemistry, Toho University, Funabashi, Chiba 274-8510, Japan. tamaki@chem.sci.toho-u.ac.jp
Abstract:
The substitution of each constituent amino acid residue of gratisin (GR) with Ala residue indicated that each side chain structure of the constituent amino acid residues affect largely the antibiotic and hemolytic activities of GR. Among them, the substitution of Pro residues at positions 5 and 5' with a cationic amino acid residues (Lys and Arg) results the high antibiotic activity and the low toxicity against human blood cells. Thus, we have found a novel position on the scaffold of GR at Pro(5,5') residues whose modification will significantly lower the unwanted hemolytic activity and enhance the desired antibiotic activity.
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