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Updated: Jun 6, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
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Fast microfluidic temperature control for high resolution live cell imaging.

Guilhem Velve Casquillas1, Chuanhai Fu, Mael Le Berre

  • 1Institut Curie, UMR 144 CNRS, Paris, 75005, France.

Lab on a Chip
|November 25, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers developed a microfluidic device for rapid temperature control in live cell imaging. This tool precisely manipulates protein activity in fission yeast (Schizosaccharomyces pombe) for studying cell dynamics.

Area of Science:

  • Cell Biology
  • Biophysics
  • Microfluidics

Background:

  • Temperature-sensitive mutations in model organisms like fission yeast (Schizosaccharomyces pombe) allow temporal control of protein function.
  • Existing temperature control devices lack the speed for real-time cellular processes.
  • Microfluidic devices offer advanced tools for cell biology research.

Purpose of the Study:

  • To develop a microfluidic device for rapid, reversible temperature switching.
  • To enable high-resolution live cell imaging with precise temporal control.
  • To investigate microtubule dynamics during the cell cycle in fission yeast.

Main Methods:

  • Fabrication of a polydimethylsiloxane (PDMS) based microfluidic device using soft-lithography.
  • Rapid reversible temperature switching between 5 °C and 45 °C in under 10 seconds.

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Last Updated: Jun 6, 2026

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  • High-resolution live cell imaging using an oil immersion objective lens.

    • Main Results:

    • The microfluidic device successfully achieved rapid temperature shifts (5 °C to 45 °C in <10 s).
    • The device facilitated high-resolution live cell imaging.
    • Demonstrated utility for studying cell cycle-dependent microtubule dynamics.

    Conclusions:

    • The developed microfluidic device provides precise temporal control over protein activity in fission yeast.
    • This technology overcomes limitations of current temperature control systems for cellular timescales.
    • Enables advanced studies of dynamic cellular processes like microtubule behavior.