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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Super-resolution fluorescence nanoscopy applied to imaging core-shell photoswitching nanoparticles and their
Zhiyuan Tian1, Alexander D Q Li, Dehong Hu
1Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
Abstract:
Using photo-actuated unimolecular logical switching attained reconstruction (PULSAR) nanoscopy, the structures of photoswitchable polymeric nanoparticles self-assembled on the surfaces of CaCl(2) crystals at the nanoscale were revealed; the photoswitching events and the locations of the photoswitchable fluorescent dyes inside the hydrophobic cores of the core-shell type polymeric nanoparticles were determined.
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