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High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

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MALDI-TOF Mass Spectrometry

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

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Mass microscopy: high-resolution imaging mass spectrometry.

Mitsutoshi Setou¹, Nobuya Kurabe

¹Department of Molecular Anatomy, Molecular Imaging Frontier Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan. setou@hama-med.ac.jp

Journal of Electron Microscopy

|November 27, 2010

Summary

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) offers high-resolution molecular localization without prior separation. This review covers MALDI-IMS principles, workflow, and biomolecular analysis applications, highlighting data complexity and statistical approaches.

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

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Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

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Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Area of Science:

Biomolecular analysis
Analytical chemistry
Mass spectrometry

Background:

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) provides spatial distribution of molecules at microscopic resolution.
MALDI-IMS enables simultaneous localization of numerous molecules without separation or purification.
MALDI-MS time-of-flight/time-of-flight (TOF/TOF) instruments are widely used for analyzing diverse biomolecules.

Purpose of the Study:

To introduce MALDI-IMS and its applications in biomolecular analyses.
To explain the principles and workflow of IMS.
To discuss related technologies and future directions in data analysis.

Main Methods:

MALDI-IMS for high-resolution molecular imaging.
Time-of-flight/time-of-flight (TOF/TOF) mass spectrometry for wide molecular weight range analysis.
Multivariate statistical analyses for handling complex spectral data.

Main Results:

MALDI-IMS allows simultaneous detection of hundreds to thousands of mass peaks per data point.
Complex IMS data necessitates advanced statistical approaches like multivariate analyses.
Future work includes developing automated molecular identification using MS(2) analysis and database searching.

Conclusions:

MALDI-IMS is a powerful technique for spatial biomolecular analysis.
Effective data analysis strategies are crucial for interpreting complex MALDI-IMS datasets.
Advancements in automated identification will enhance future MALDI-IMS applications.