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Updated: Jun 6, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Orientated glyco-macroligand formation based on site-specific immobilization of O-cyanate chain-end functionalized
Satya Nandana Narla1, Xue-Long Sun
1Department of Chemistry, Cleveland State University, 2121 Euclid Avenue SI 313, Cleveland, OH 44115, USA.
Abstract:
We report an oriented glyco-macroligand formation and its glyco-affinity capturing and glycoarray application by combining an O-cyanate chain-end functionalized glycopolymer with commercially available amine-functionalized silica gel and glass slides via isourea bond formation. The O-cyanate chain-end functionalized glycopolymer was synthesized via cyanoxyl-mediated free-radical polymerization (CMFRP) in a one-pot fashion. The oriented glyco-macroligand formation was conducted under mild aqueous conditions at room temperature and was confirmed with fluorescence imaging after specific lectin binding, and will find important biomedical applications such as in biosensors and glycoarrays. In addition, the O-cyanate chain-end functionalized glycopolymer can also be used for site-specific polymer-protein conjugation.
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