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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
Flow cytometry resonance energy transfer suggests an association between low-affinity interleukin 2 binding sites and
A Harel-Bellan1, P Krief, L Rimsky
1Laboratory of Immunoregulation, National Cancer Institute, Frederick, MD 21701.
The Biochemical Journal
|May 15, 1990
Abstract:
Using flow cytometry energy transfer we have studied the sterical proximity of interleukin 2 receptors and the heavy chain of the major histocompatibility complex at the surface of normal lymphocytes. Our data suggest that class I molecules may be part of a low-affinity interleukin 2 receptor multimolecular complex, where the MHC class I heavy chain is in close proximity to the actual interleukin 2 binding site, in contrast to the light chain (beta 2-microglobulin).

