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Published on: September 11, 2015
Influence of novel nano-titanium implant surface on human osteoblast behavior and growth
Stefano Tetè1, Filiberto Mastrangelo, Raimondo Quaresima
1Department of Oral Science, University G. d'Annunzio, Chieti, Italy. tete@unich.it/filibertomastrangelo@hotmail.com
Purpose:
The aim of the study is to investigate human osteoblast-like cell behavior and growth in the presence of 3 different titanium implant surfaces.
Materials:
Human stem cells were first obtained and then sorted by fluorescence-activated cell sorter from mesenchymal stem cell clusters of human dental papilla. The obtained human dental papilla stem cells were induced to differentiate into osteoblast-like cells and were then analyzed by reverse transcriptase polymerase chain reaction and Western blot analyses. The cells proliferated and were cultured onto 3 different titanium discs (sandblasted, sandblasted and large-grit acid-etched, and full contact coverage [FCC]) and analyzed by scanning electron microscope.
Results:
In all analyses samples, a high cell activity was observed, with typical osteoblast mature morphostructural response on rough surface. The high number of osteoblast-like cells was found on titanium FCC discs. At the same time, scanning electron microscope analysis confirmed the high biocompatibility of this surface.
Conclusion:
The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.
