Related Experiment Video
Updated: Jun 6, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
[Effects of antisense oligodeoxynucleotides on vascular endothelial growth factor expression in Lewis lung carcinoma
Ying Wang1, Chunyan Lin, Chunyan Li
1Department of Laboratory Diagnosis, The First Clinical College, Harbin Medical University, Harbin, Heilongjiang 150001, P.R.China.
Background:
Vascular endothelial growth factor (VEGF) can specially target the vascular endothelial cells so as to enhance their proliferation and angiogenesis. The aim of this study is to investigate the inhibition effects of VEGF antisense oligodeoxynucleotides (VEGF ASODN) on VEGF expression of Lewis lung carcinoma cells.
Methods:
The phosphorothioate-modified and liposome-encapsulated VEGF ASODN, VEGF SODN were added into the medium of Lewis lung carcinoma cells respectively. VEGF mRNA and protein were detected by RT-PCR and immunohistochemistry. And the proliferation of vascular endothelial cells treated with supernatants was determined.
Results:
VEGF ASODN could significantly inhibit the expression of VEGF mRNA and protein expression in Lewis lung carcinoma cells, as well as the proliferation of endothelial cells.
Conclusions:
It is hopeful that VEGF ASODN may become a new antiangiogenesis therapy strategy in lung cancer.
Insights
Vascular endothelial growth factor antisense oligodeoxynucleotides (VEGF ASODN) effectively inhibit VEGF expression in lung cancer cells. This suggests VEGF ASODN as a potential antiangiogenesis therapy for lung cancer.
Area of Science:
- Molecular Biology
- Cancer Research
- Biochemistry
Context:
- Vascular endothelial growth factor (VEGF) promotes tumor angiogenesis and proliferation.
- Lewis lung carcinoma cells express VEGF, contributing to tumor growth.
- Targeting VEGF is a key strategy in anti-cancer therapy.
Purpose:
- To investigate the inhibitory effects of VEGF antisense oligodeoxynucleotides (VEGF ASODN) on VEGF expression.
- To evaluate the impact of VEGF ASODN on Lewis lung carcinoma cells and endothelial cell proliferation.
Summary:
- VEGF ASODN, both phosphorothioate-modified and liposome-encapsulated, were applied to Lewis lung carcinoma cells.
- VEGF mRNA and protein levels were significantly reduced by VEGF ASODN treatment.
- Endothelial cell proliferation was inhibited when treated with supernatants from VEGF ASODN-treated cells.
Impact:
- VEGF ASODN demonstrates significant potential as an anti-angiogenesis agent.
- This study supports VEGF ASODN as a promising therapeutic strategy for lung cancer treatment.
- Further research into VEGF ASODN could lead to novel lung cancer therapies.
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
02:49Cancer Therapies
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...