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Updated: Jun 6, 2026

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Introducing predetermined mutations throughout a target gene using TDEM (transposon-directed base-exchange

Yun Cheol Kim1

  • 1Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, USA. kyc8402@ucla.edu

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2010
PubMed
Summary

Transposon-directed base-exchange mutagenesis (TDEM) precisely modifies genes by deleting and inserting DNA sequences. This controllable method minimizes unwanted mutations, aiding protein structure and function studies.

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