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Related Concept Videos

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

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Related Experiment Video

Updated: Jun 6, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

The post-transcriptional operon.

Scott A Tenenbaum1, Jan Christiansen, Henrik Nielsen

  • 1College of Nanoscale Science and Engineering, Nanoscale Constellation, University at Albany-SUNY, Rensselaer, NY, USA. stenenbaum@albany.edu

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2010
PubMed
Summary
This summary is machine-generated.

The post-transcriptional operon model coordinates protein expression using RNA regulation. This model helps identify new cellular pathway participants and understand cellular responses.

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Post-transcriptional Control

Background:

  • Gene expression is regulated at multiple levels.
  • Post-transcriptional regulation fine-tunes protein output.
  • The post-transcriptional operon model (PTO) provides a framework for understanding coordinated mRNA regulation.

Purpose of the Study:

  • To define the concept and utility of the post-transcriptional operon model.
  • To highlight the role of RNA-binding proteins and small non-coding RNAs in co-regulating mRNA.
  • To demonstrate the application of the PTO model in analyzing global mRNA functionality.

Main Methods:

  • Integration of data from various high-throughput techniques.
  • Analysis of RNA-binding protein and small non-coding RNA interactions with mRNA.
  • Utilizing methods such as RIP-Chip, CLIP-Chip, miRNA profiling, and ribosome profiling.

Main Results:

  • The PTO model effectively describes co-regulated sets of monocistronic mRNAs.
  • Protein expression is coordinated post-transcriptionally through this model.
  • Established examples showcase the model's power in biological discovery.

Conclusions:

  • The post-transcriptional operon model is a valuable tool for systems biology.
  • It facilitates the identification of novel components within cellular pathways.
  • The PTO model enhances the understanding of complex cellular responses and gene networks.