Microfluidic immobilization of physiologically active Caenorhabditis elegans

Area of Science:

• Biomedical Engineering
• Developmental Biology
• Microfluidics

Background:

• Studying dynamic cellular processes in Caenorhabditis elegans requires precise immobilization.
• Existing methods often use anesthetics or cooling, which can affect physiological states.

Purpose of the Study:

• To present a novel microfluidic device for mechanical immobilization of Caenorhabditis elegans.
• To enable advanced in vivo imaging and microsurgery of the worm in its active state.

Main Methods:

• Fabrication involves molding elastomeric layers on silicon wafers and thermal bonding.
• The device uses a flexible membrane to mechanically restrain the worm.
• Operation requires a single syringe and external valve, adaptable to standard microscopes.

Main Results:

• The device achieves linear orientation and complete motion restraint of C. elegans.
• It supports various in vivo studies including cell division, migration, aging, and regeneration.
• Applications include laser microsurgery, Ca2+ imaging, and 3D microscopy.

Conclusions:

• This anesthetic-free microfluidic device offers a versatile and accessible platform for C. elegans research.
• It preserves the worm's physiological state, enhancing the accuracy of in vivo studies.
• The system's ease of use and fabrication facilitates its adoption in diverse laboratories.