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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Intact skin analysis by desorption electrospray ionization mass spectrometry
Mária Katona1, Júlia Dénes, Réka Skoumal
1Cell Screen Applied Research Centre, SemmelweisUniversity, Budapest, Hungary.
The Analyst
|December 7, 2010
Summary
Desorption Electrospray Ionization (DESI) enables real-time skin analysis for drug kinetics and oxidative stress monitoring. This innovative technique shows promise in forensics, pharmacology, and clinical chemistry applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Pharmacology
Background:
- In vivo skin analysis presents challenges for real-time molecular monitoring.
- Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) offers a potential solution for direct skin analysis.
Purpose of the Study:
- To characterize in vivo skin analysis using pneumatically assisted electrospray directly on human fingertips.
- To develop a novel method for real-time quantification of oxidative stress via skin analysis.
- To evaluate the potential applications of DESI-MS in various scientific fields.
Main Methods:
- Direct pneumatically assisted electrospray applied to healthy human volunteers' fingertips.
- Utilized a high ohmic resistor to prevent electric shock during analysis.
- Performed pharmacokinetic studies on a rat model, monitoring ketamine kinetics and N,N'-dimethylthiourea (DMTU) oxidation.
- Compared in vivo DESI-MS results with off-line High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) blood analysis.
Main Results:
- Negative ion DESI-MS spectra revealed various biogenic carboxylic acids.
- Demonstrated differential analysis kinetics for external and excreted molecules, exemplified by nicotine and cotinine.
- Ketamine pharmacokinetics monitored by DESI-MS aligned with HPLC-MS blood analysis.
- The ratio of DMTU to its oxidized form on skin effectively quantified experimentally induced diabetes and chronic oxidative stress.
Conclusions:
- DESI-MS is a viable technique for in vivo skin analysis, offering insights into drug pharmacokinetics.
- A novel method for real-time oxidative stress quantification from skin surface was developed.
- The technique shows significant potential for applications in forensics, pharmacology, and clinical chemistry.
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