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Published on: August 7, 2014
Body fluid proteomics: Prospects for biomarker discovery
Sung-Min Ahn1, Richard J Simpson
1Joint ProteomicS Laboratory, Ludwig Institute for Cancer Research, Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital Parkville, Victoria, Australia; Gachon Institute for Systems Medicine, Gachon University of Medicine and Science, Incheon, Korea.
Proteomics. Clinical Applications
|December 8, 2010
Summary
Identifying disease signatures in body fluids is key for early detection. This study explores proteomics strategies and albumin
Area of Science:
- Biochemistry
- Proteomics
- Biomarker Discovery
Background:
- Cellular signaling pathway perturbations, often due to genetic aberrations, lead to disease.
- Altered protein profiles in tissue interstitial fluid (TIF) reflect these cellular changes.
- Mapping these protein profiles (
Purpose of the Study:
- To discuss body fluid use in biomarker discovery.
- To address challenges in proteomics, including protein dilution and depletion strategies.
- To present a model for understanding biomarker research complexity, using albumin and Alzheimer's disease as examples.
Main Methods:
- Review of proteomics strategies for analyzing body fluids.
- Discussion of protein dilution from TIF to circulatory system (1000-1500-fold).
- Analysis of major serum protein depletion techniques, focusing on albumin.
Main Results:
- Major serum protein depletion can remove low-molecular-weight proteins/peptides.
- Albumin plays a multifaceted role in biomarker research, exemplified by its involvement in Alzheimer's disease.
- A systems-level understanding is crucial for effective biomarker research.
Conclusions:
- Biomarker discovery requires advanced proteomics to overcome challenges like protein dilution and depletion limitations.
- A systems-level approach, considering interactions like albumin binding, is essential for personalized medicine.
- Understanding protein signatures in body fluids aids disease detection and monitoring.
