[Effect of reactive oxygen species during the leukemogenic process associated with exposure to benzene]
Yi-min Liu1, Yong-sheng Li, Xu-dong Li
1Guangzhou Occupational Disease Prevention & Treatment Centre, Guangzhou, 510620, China.
Objective:
To study the effect of reactive oxygen species (ROS) during the leukemogenic process associated with exposure to benzene.
Methods:
HL-60 was treated with 3 micromol/L benzoquinone (BQ). Generation of ROS in cells was measured by DCFH-DA method. For proliferation assays,cells were stained with alamar blue dye and counted.
Results:
ROS production and the proliferation of cell were all increased in BQ-treated cells (13.10 +/- 0.15, 185% +/- 30.00%) as compared with control cells (11.32 +/- 0.09, 100% +/- 0.00%) (P < 0.05); The addition of catalase just before BQ addition reduced ROS generation to basal levels and decreased the growth of cell (P < 0.05).
Conclusion:
ROS may play an important role in the process of proliferation of HL-60 cells induced by BQ.
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