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Related Concept Videos

Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion01:22

Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion

The kidneys maintain homeostasis through filtration, reabsorption, and secretion. Tubular reabsorption and secretion are crucial in forming urine and regulating electrolytes, water balance, and waste elimination.Tubular Reabsorption and Secretion ProcessesTubular reabsorption is the process that reclaims essential substances such as electrolytes, glucose, amino acids, and water from the glomerular filtrate back into the bloodstream. This is achieved through passive and active transport...
Regulation of Sodium and Potassium01:26

Regulation of Sodium and Potassium

The regulation of sodium and potassium ion concentrations in the human body is a complex process governed primarily by hormones such as aldosterone, antidiuretic hormone (ADH), and atrial natriuretic peptide (ANP).
Sodium Regulation
Sodium ions make up approximately 90% of extracellular cations, with a normal blood plasma concentration of 136–148 mEq/L. A decrease in blood volume and pressure triggers the release of renin from granular cells in the juxtaglomerular complex (JGC), primarily in...
Reabsorption and Secretion in the DCT and Collecting Duct01:26

Reabsorption and Secretion in the DCT and Collecting Duct

The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal part of the DCT, along with the...

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Related Experiment Video

Updated: Jun 6, 2026

Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)
08:54

Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)

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[Ion channel mechanism of regulatory volume decrease in human epithelial cells].

Lu Ping Shi1, Yi Min Zang, Xiao Li Hou

  • 1Department of Physiology, Capital Medical University, Beijing 100069, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Human intestine cells exhibit regulatory volume decrease (RVD) via parallel activation of chloride and intermediate-conductance, calcium-activated potassium (IK) channels. This study elucidates the ion channel mechanisms underlying RVD in intestinal cells.

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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
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Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

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Related Experiment Videos

Last Updated: Jun 6, 2026

Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)
08:54

Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)

Published on: August 9, 2024

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
05:42

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Published on: January 7, 2019

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
11:32

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

Area of Science:

  • Cell biology
  • Physiology
  • Molecular biology

Context:

  • Cell volume regulation is crucial for cellular function.
  • Human intestine cells undergo regulatory volume decrease (RVD) when exposed to hypotonic stress.
  • The specific ion channel mechanisms involved in intestinal cell RVD require further elucidation.

Purpose:

  • To investigate the regulatory volume decrease (RVD) process in human intestine cells.
  • To identify the ion channel mechanisms, particularly potassium and chloride channels, involved in intestinal cell RVD.
  • To determine the specific subtype of calcium-activated potassium channel involved.

Summary:

  • Human intestine cells demonstrate RVD when exposed to hypotonic solutions.
  • RVD was inhibited by chloride channel blocker NPPB and potassium channel blocker TEA, indicating involvement of both.
  • The study identified an intermediate-conductance, calcium-activated potassium channel (IK) as the specific potassium channel subtype responsible for RVD, confirmed by clotrimazole sensitivity and RT-PCR expression analysis.

Impact:

  • This research clarifies the ion channel-dependent mechanisms of RVD in human intestine cells.
  • Findings highlight the parallel activation of chloride and IK channels in maintaining cell volume homeostasis.
  • Provides a foundation for understanding cellular responses to osmotic stress in the gastrointestinal tract.