Towards ETEM serial crystallography: Electron diffraction from liquid jets
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Updated: Jun 6, 2026

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
Published on: February 28, 2019
D P Deponte1, J T McKeown, U Weierstall
1Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287, USA. Daniel.deponte@desy.de
Abstract:
A sufficiently thin column of liquid was produced to permit penetration with a 200 keV electron beam as evidenced by the observation of diffraction rings due to the intermolecular spacing of the liquid samples. For liquid thickness below 800 nm, the diffraction rings became visible above the inelastic background. Studies were carried out in the environmental chamber of a transmission electron microscope using water and isopropanol.

